1046 E Chestnut St Louisville, KY 40204

This is an approximately 100,000 square foot life science facility available for lease in Louisville, Kentucky. Situated on 7.5 acres, the building features infrastructure tailored to pharmaceutical, medical device, biotech, diagnostic, and clean manufacturing users. The property previously housed multiple pharmaceutical and medical device operations and is currently configured with five wet laboratories and fifteen clean rooms, the majority of which are ISO Class 7 (Class 10,000), with the balance ISO Class 6 (Class 1,000). The facility includes an FDA-certified, fully heated and air-conditioned warehouse, along with a 400 SF walk-in medical freezer. Originally constructed as a garment factory around the time of the U.S. Civil War, the building combines historic industrial character with modern life-science functionality. The site offers approximately 400 on-site parking spaces and excellent access to major transportation corridors. The property can accommodate a range of configurations, from dedicated lab and clean-room suites to full-building occupancy and is suitable for users requiring FDA-compliant manufacturing, testing, R&D, or controlled-environment production. It significantly reduces the time, cost, and uncertainty typically associated with lab and clean-room development and offers a compelling cost advantage without sacrificing regulatory readiness. This is an ideal opportunity for organizations seeking a move-in-ready, regulated facility with existing approvals and flexibility for growth.